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Figure 3 | Cell Communication and Signaling

Figure 3

From: Agent-based modeling of autophagy reveals emergent regulatory behavior of spatio-temporal autophagy dynamics

Figure 3

Single cell analysis of autophagic flux in MCF7 cells. MCF7 cells stably co-expressing GFP-Rab7 (green) and mCherry-LC3 (red) were submitted to FM and ND conditions for 3 hours, in the absence and presence of BAF (100 nM). A Merged image of a typical cell under FM conditions. B-C Individual green and red channels of (A) are shown as inverted black-white images and corresponding segmented masks. D Merged image of a typical cell under FM conditions with BAF inhibition of lysosomes. E-F Individual green and red channels of (D) are shown as inverted black-white images and corresponding segmented masks. A-F Green arrows indicate Rab7(+)/LC3B(-) endo-lysosomes, red arrows indicate Rab7(-)/LC3B(+) autophagosomes, and yellow arrows indicate Rab7(+)/LC3B(+) autolysosomes. G Histogram displaying APs, LYs, ALs vesicle numbers for the different conditions (left), and list of the corresponding mean values (right). H Histogram displaying APs, LYs, ALs vesicle sizes for the different conditions (left), and list of the corresponding mean values (right). Number of analyzed cells: FM 8, FM/BAF 4, ND 14, ND/BAF 7.

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