Figure 6

Matrigel invasion of MDA-MB-231 cells is inhibited by BDP5290. (A) MDA-MB-231 human breast cancer cells were plated in 96 well plates, then 24 hours later a ~800 μM scratch wound was created. Matrigel was overlayed for 1 hour, then images were acquired after 0 and 24 hours in the presence of DMSO vehicle or 3 μM BDP5290. (B) Relative wound closure of MDA-MB-231 cells imaged up to 24 hours at 3 hour intervals in the presence of indicated BDP5290 concentrations. (C) Relative wound closure of MDA-MB-231 cells imaged up to 24 hours at 3 hour intervals in the presence of indicated Y27632 concentrations. (D) Relative wound closure of MDA-MB-231 cells at 24 hours in the presence of indicated BDP5290 or Y27632 doses. All results are shown as mean ± standard error of n = 4 independent replicates. (E) Alamar Blue metabolism of MDA MB 231 cells after 24 hours in the presence of DMSO vehicle, indicated concentrations of BDP5290 or 10 μM Y27632. Readings were compared to the Alamar Blue metabolism in DMSO treated cells that were used as the viability standard of 100% for each replicate experiment. All results are shown as mean ± standard error of 3 independent replicates.