BtpA or variant | Localisation of GFP-BtpA in filaments (% of transfected cells ± SD) | Protection of microtubules after NC treatment(% of cells with BtpA arranged in filaments)a | Induction of filopodia(% of cells with BtpA arranged in filaments)b |
---|---|---|---|
BtpA | 90.5 ± 1.36 | 73,8 ± 3.45 | 75.9 ± 0.26 |
BtpA W213S | 0 | 0 | 0 |
BtpA W213A | 90.1 ± 3.86 | 75,6 ± 5,00 | 0 |
BtpA W213F | 81.9 ± 1.81 | 87,1 ± 5,36 | 0 |
BtpA E217D | 1.3 ± 0.78 | 83.9 ± 9.89 | 0 |
BtpA E217A | 52.6 ± 6.67 | 0 | 0 |
BtpA I226S | 14.4 ± 1.93 | 76,1 ± 3,66 | 69.7 ± 3.5 |
BtpA G183A | 0 | 0 | 0 |
BtpB or variant | Protection of microtubules after NC treatment (% of cells with) c | Induction of filopodia | |
Diffuse BtpB pattern | Punctate BtpB pattern | ||
BtpB | 24.5 ± 0.71 | 82,2 ± 1.59 | 0 |
BtpB W263S | 0 | 0 | 0 |
BtpB E267A | 0 | 0 | 0 |