Figure 9

Immunohistologic analysis of wild type or itr1aΔ itr3cΔ mutant infected mouse brain. Antibody mediated immunofluorescent staining were performed using brain sections from mouse infected with the wild type H99 or the itr1aΔ itr3cΔ mutant strain. The stained sections were analyzed by confocal microscopy followed by 3D reconstructions of images. Tissue sections were stained for the host cell nuclei (DAPI, blue), fungal GXM (FITC-labeled GXM antibody 18B7, Green), host GFAP (astrocyte marker, red) or host Iba-1 (macrophage/microglia marker, red). Scale bar: 180 μm.