Figure 1
From: TCR-triggered extracellular superoxide production is not required for T-cell activation
TCR stimulation induces extracellular superoxide release in primary human T cells. (A) Primary human T cells were stimulated with CD3xCD28- or isotype-coated microbeads. Superoxide production was measured with Diogenes assay at 5 min intervals. The values indicate the increase in luminescence in CD3xCD28- relative to isotype-stimulated samples. The data show the mean from 16 independent experiments. (B) Primary human T cells were stimulated for 10 min with CD3xCD28-coated microbeads alone or in the presence of either SOD, catalase or ascorbate. Superoxide production was measured with Diogenes assay. The values indicate the luminescence signal normalized to stimulated controls. The data show the mean from at least 4 independent experiments.