Figure 8

RelB suppresses CXCL8, but not CCL5, in BEAS- 2B cells. Cells were transfected with siRNA against RelB (siRELB) or non-targeting control siRNA (siNT). Transfected cells were exposed to 20 μM 1-NP, 1-AP, or vehicle (DMSO) alone (A-D) or 10 μg/ml Poly I:C or vehicle (water) alone (E and F). CXCL8 (A) and CCL5 (B) gene expression were measured after 6 h by real-time PCR. CXCL8 (C and E) and CCL5 (D and F) protein levels in the medium were measured by ELISA after 18 h exposure as described under “Materials and methods”. Efficiency of transfection was assessed by Western blotting (G). The results are expressed as mean ± SEM (A/B: n = 1 (triplicate determinations); C-F: n ≥ 3; G: representative blot, n = 3) *Significantly different from unexposed controls; †Significantly different from cells transfected with non-targeting siRNA.