Figure 5

AhR and Arnt differentially regulate CXCL8 and CCL5 responses as well as p65 phosphorylation in Poly I: C- exposed BEAS- 2B cells. Cells were transfected with siRNA against p65 (siP65), AhR (siAHR), Arnt (siARNT) or non-targeting control siRNA (siNT), and exposed to 10 μg/ml Poly I:C. Intracellular protein levels of total and phospho-p65 (Ser536) as well as AhR and Arnt were detected by Western blotting after 2 and 4 h exposure, as described under “Materials and methods”. The figure displays representative blots (A), as well as relative changes in phospho-p65 compared to total p65 quantified by densitometric analysis of the Western blots (B). CXCL8 (C and E) and CCL5 (D and F) protein levels in the medium were measured by ELISA after 18 h exposure, as described under “Materials and methods”. The results are expressed as mean ± SEM (n ≥ 3). *Significantly different from unexposed controls; †Significantly different from cells transfected with non-targeting siRNA.