Figure 5

Intracellular domain of TrkB was required for NRG1 induced NR2B activation. (A) BDNF treatment increased TrkB activation in cortical neurons transfected with full-length TrkB (TrkB-FL), but not in neurons transfected with TrkB lacking intracellular domain (TrkB ∆ ICD). The upper panel shows a representative autoradiogram of pTrkB and TrkB, and the lower panel represents normalized pTrkB values. Data represent mean ± SEM. (n = 3). *P < 0.05 vs control; ^$P < 0.05 vs BDNF treatment (B) NRG1 failed to activate NR2B in neurons transfected with TrkB ∆ ICD. The upper panel shows a representative autoradiogram of pNR2B and NR2B, and the lower panel represents normalized pNR2B values. Results are mean ± SEM. (n = 3) *P < 0.05 vs TrkB-FL; ^$P < 0.05 vs NRG1 + TrkB-FL. (C) Immunoprecipitation (IP) with the anti-TrkB antibody or control IgG antibody followed by blotting with the anti-ErbB4 or anti-TrkB antibody was performed in NRG1-treated cortical neurons transfected with TrkB lacking intracellular domain (TrkB ∆ ICD).