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Figure 3 | Cell Communication and Signaling

Figure 3

From: TrkB interacts with ErbB4 and regulates NRG1-induced NR2B phosphorylation in cortical neurons before synaptogenesis

Figure 3

PLCγ mediates NR2B activation by NRG1. (A) NRG1-induced PLCγ activation is TrkB dependent. The upper panel shows a representative autoradiogram of pPLCγ and PLCγ, and the lower panel represents normalized pPLCγ values. (Bi) Immunoblot analysis of NRG1-induced activation of PLCγ and NR2B in presence of U73122. U73122 inhibited NRG1-increased phosphorylation of (Bii) PLCγ and (Biii) NR2B. Data represent mean ± SEM. (n = 5). *P < 0.05 vs control; $P < 0.05 vs NRG1 treatment. (Ci) Endogenous PLCγ was decreased after PLCγ siRNA transfection compared to scrambled siRNA. The upper panel shows a representative autoradiogram of PLCγ and β-actin, and the lower panel represents normalized PLCγ values. Data represent mean ± SEM. (n = 3). *P < 0.05. (Cii) NRG1-induced NR2B activation was reduced in PLCγ siRNA transfected cultures. The upper panel shows a representative autoradiogram of pNR2B and NR2B, and the lower panel represents normalized pNR2B values. Data represent mean ± SEM. (n = 3). *P < 0.05 vs scrambled siRNA group; $P < 0.05 vs NRG1 treated scrambled siRNA group. (D) Immunoblotting analysis of NRG1-induced PKCzeta activation in isolated primary cortical neurons. The upper panel shows a representative autoradiogram of pPKCzeta and PKCzeta, and the lower panel represents normalized pPKCzeta values. Data represent mean ± SEM. (n = 3). *P < 0.05 vs control; $P < 0.05 vs NRG1 treatment.

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