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Figure 3 | Cell Communication and Signaling

Figure 3

From: The tumor marker Fascin is induced by the Epstein-Barr virus-encoded oncoprotein LMP1 via NF-κB in lymphocytes and contributes to their invasive migration

Figure 3

CTAR2 is the major site of LMP1-mediated Fascin induction. (A) Scheme of LMP1 and its signaling domains, the C-terminal activating regions 1 (CTAR1; aa 194–231) and 2 (CTAR2; aa 351–386). Mutations of the P(204)xQxT consensus motif to AxAxA in CTAR1 and deletion of aa 371–386 in CTAR2 are indicated. Signaling pathways induced by LMP1 are shown in rectangular boxes, adaptor molecules in rounded boxes. can. indicates canonical; noncan., noncanonical. (B) qPCR of Fascin transcripts 48 h after transfection of Jurkat cells with wt-LMP1 (20 μg pCMV-HA-LMP1), LMP1 mutants (40 μg pCMV-HA-LMP1(AAA), 20 μg of pCMV-HA-LMP1-Δ371-386) and HTLV-1/Tax (40 μg pcTax-1) in comparison to mock-transfected cells (100 μg pcDNA3). Total transfected DNA was adjusted to 100 μg with pcDNA3. Copy numbers were normalized to those of ACTB and on mock-transfected cells. Mean values +/− standard error (SE) are given. (C) Detection of Fascin and LMP1 by immunoblot after transient transfection of Jurkat cells with the constructs described in (B). Fascin expression was quantitatively evaluated by densitometry and normalized on LMP1 protein expression. The mean of three independent experiments +/−SE is shown. **indicates P < 0.01. n.s., not significant.

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