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Figure 4 | Cell Communication and Signaling

Figure 4

From: Chk1 activity is required for BAK multimerization in association with PUMA during mitochondrial apoptosis

Figure 4

Interaction between Chk1 and pro- and anti-apoptotic Bcl-2 family proteins. (A) Immunoprecipitation was performed with anti-Chk1 ab (Santacruz) and immunoblotted for BAK (top panel) and Chk1 (bottom panel). Similar immunoprecipitations were performed with anti-Chk1 ab and immunoblotted for PUMA (B) and Bcl-xL (C). In all immunoprecipitation reactions 5% of mitochondrial extracts was used as input. The non-UV mitochondrial extract was used in all IgG control immunoprecipitation reactions. (D) Chk1 levels were assessed in mitochondrial extracts of both HC1116-WT BAK and HT1080 cells following UV treatment or without UV. CoxIV represents equal loading.

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Cell Communication and Signaling

ISSN: 1478-811X