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Figure 3 | Cell Communication and Signaling

Figure 3

From: Identifying USPs regulating immune signals in Drosophila: USP2 deubiquitinates Imd and promotes its degradation by interacting with the proteasome

Figure 3

USP2 and USP34 regulate immune signalling in vivo . Quantitative analysis of Dpt, AttA, Drs and IM1 mRNAs by RT-qPCR. A. Histograms present the fold activation of the indicated gene mRNA in Usp2 (c564 > Usp2-IR) or Usp34 (c564 > Usp34-IR) silenced flies compared to control flies (c564Gal4/+) raised in similar conditions. B. Dpt and AttA induction in double silenced flies (c564 > Usp2-IR, Imd-IR or c564 > Usp34-IR, Imd-IR) expressed as a % of activity of Usp-only silenced flies (c564 > Usp2-IR or c564 > Usp34-IR, respectively). C,D. Activation of Dpt and AttA in Usp2- (C) or Usp34 (D) -silenced flies, in combination or not with an Imd-silencing transgene-, at 3 hours post E. coli infection, expressed as a % of activity compared to infected control flies (c564/+). E. Activation of AttA, Drs and IM1 in Usp34-silenced flies at 3 and 24 hours post M. luteus infection expressed as a % of activity compared to infected control flies (c564/+) (100% is the maximal activation observed in control flies at 3 hours post infection for AttA, and IM1 and at 24 hours post-infection for Drs). A-E. One representative experiment out of three is shown. Error bars indicate standard deviation between technical triplicates. Significant differences (t-test), p < 0.05 (*), p < 0.02 (**), p < 0.001 (***).

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