Figure 2

Overexpressing USP2 or USP34 suppresses fly immunity in vivo. A-D. Flies were heat shocked 12 hours before infection. A. Quantitative analysis of Dpt mRNAs levels by RT-qPCR at 3, 6, 9 and 12 hours post infection with E. coli. B. Forty heat-shocked flies of 5 days old were infected with E. cloacae and their survival kinetics was followed over 24 hours. The mutant key is used as a Gram-negative bacteria sensitive control (non-heat shocked flies). C. Quantitative analysis of Drs mRNAs by RT-qPCR at 12 and 24 hours post infection with M. luteus. D. Forty flies of 5 days old were infected with E. faecalis 6 hours following heat shock treatment and their survival kinetics was followed over 48 hours. The mutant w¹¹¹⁸;Dif,Key is used as a Gram-positive bacteria sensitive control (non-heat shocked flies). A,C: Results are expressed as the fold induction level compared to the level of Dpt (A) or Drs (C) mRNAs in non-infected flies. Error bars indicate standard deviation between technical replicates. One representative experiment out of three is shown. Significant difference: p < 0.05 (*), p < 0.002 (**) or p < 0.0001 (***) compared to HspGal4/+control flies (Student’s-t-test). B,D: Results are expressed as % of surviving flies. One representative experiment out of three is shown. Significant difference: p < 0.002 (**) or p < 0.0001 (***) compared to HspGal4/+control flies (Log-rank (Mantel-Cox) test). A-D. Flies’ genotypes are: w¹¹¹⁸;HspGal4/+ (HspGal4/+), w¹¹¹⁸;HspGal4/+; P{EPgy2}ash2EY03971/+ (Hsp > Usp34), w¹¹¹⁸;HspGal4/+;UAS-Usp2/+ (Hsp > Usp2), w¹¹¹⁸;key¹ (key) and w¹¹¹⁸; Dif¹, key¹(Dif,key).