Caspase-1 controls production of IL-1β in mast cells. (A) wt BMMCs were primed with 1 μg/ml LPS for 3.5 h and then left untreated or stimulated with the indicated concentrations [mM] of ATP for 1 h; vehicle (DMSO) or caspase-1 inhibitor (YVAD-CHO) was added 1 h prior to stimulation with ATP. TCL and SN were probed for IL-1β by ELISA (n = 5) (left panel). BMMCs were stained with Pi and analyzed by FACS (n = 3) (right panel). (B) Treatment as in (A); TCL of wt BMMCs was probed for IL- β by ELISA (n = 5). (C) Treatment as in (A); transcripts were analyzed by qPCR (n = 3). Shown are means and SD of replicates of one representative experiment each. Statistical analysis of n independent experiments by LMM; FDR-corrected p-values: * < 0.05, ** < 0.005, and *** < 0.0005.