Figure 4

Gα o enhances cell growth suppression induced by Necdin. 293T cells were transfected with plasmids encoding various types of Gα (0.5 μg) and FLAG-Necdin (1 μg). To identify transfected cells, we co-transfected with the pEGFP (100 ng). After 24 h of transfection, cells were labeled with 10 μM BrdU for 12 h and stained with antibodies against BrdU and GFP. The extent of BrdU incorporation was assessed in GFP-positive cells. Data are shown as the average ± SE of at least three independent experiments. WT and Q205L indicate wild-type and constitutively active mutant of Gαo, respectively. *, p < 0.001.