Table 4 Tested and working antibodies for analysis of cell cycle and apoptosis by western blot methodology.
From: A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells
Antibody name | Company and Cat # | Dilution and expected size (KDa). |
|---|---|---|
P53 | sc-126(DO-1) mouse | 1:1000 5%milk/TBS. 53 KDa |
P19ARF | R562 Abcam Rabbit | 1:1000 5%milk/TBS 25 Kda (30-50 μg) |
P16INK4a | M-156 (sc-Rabbit) | 1:1000 5%milk/TBS 16 Kda |
Rb | C15 (sc rabbit) | 1:1000 5%milk/TBS 105 KDA |
P107 | C18 (sc Rabbit) | 1:1000 5%milk/TBS. 107 KDa |
Cyclin A | C19 (sc-rabbit) | 1:1000 5%milk/TBS 54 Kda |
Cyclin A1 | H-230 (sc rabbit) | 1:1000 5%milk/TBS 54 Kda |
Cyclin A2 | Epitomics E399 (rabbit) | 1:500 5%milk/TBS 54 Kda |
Cyclin B1 | Sc-245 (mouse) | 1:1000 5%milk/TBS 60 Kda |
Cyclin D1 | H-295 (sc rabbit) | 1:1000 5%milk/TBS 34 KDa |
Cyclin D2 | M20 (sc rabbit) | 1:1000 5%milk/TBS 34 KDa |
Cyclin E | M20 (sc rabbit) | 1:1000 5%milk/TBS 53 KDa |
E2F1 | Sc-251 (sc mouse) | 1:1000 5%milk/TBS 55 KDa |
P21CIP | F5 (sc mouse) | 1:1000 5%milk/TBS 21 KDa |
P27KIP | (M-197) sc776 rabbit | 1:1000 5%milk/TBS 27 KDa |
CDK4 | C22 (sc rabbit) | 1:1000 5%milk/TBS 34 KDa |
CDK2 | H-298 (sc rabbit) | 1:1000 5%milk/TBS 34 KDa |
PCNA | PC-10 (sc. Mouse) | 1:1000 5%milk/TBS 36 KDa |
MDM2 | Abcam 2A10 (mouse) | 1:1000 5%milk/TBS 80 KDa(30-50 μg) |
Cleaved Casapse 3 | Cell sign (rabbit) #9664 | 1:1000 5%milk/TBS 18 KDa |
Cdc27 | C4 (sc mouse) | 1:1000 5%milk/TBS 102 KDa |