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Table 4 Tested and working antibodies for analysis of cell cycle and apoptosis by western blot methodology.

From: A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

Antibody name Company and Cat # Dilution and expected size (KDa).
P53 sc-126(DO-1) mouse 1:1000 5%milk/TBS. 53 KDa
P19ARF R562 Abcam Rabbit 1:1000 5%milk/TBS 25 Kda (30-50 μg)
P16INK4a M-156 (sc-Rabbit) 1:1000 5%milk/TBS 16 Kda
Rb C15 (sc rabbit) 1:1000 5%milk/TBS 105 KDA
P107 C18 (sc Rabbit) 1:1000 5%milk/TBS. 107 KDa
Cyclin A C19 (sc-rabbit) 1:1000 5%milk/TBS 54 Kda
Cyclin A1 H-230 (sc rabbit) 1:1000 5%milk/TBS 54 Kda
Cyclin A2 Epitomics E399 (rabbit) 1:500 5%milk/TBS 54 Kda
Cyclin B1 Sc-245 (mouse) 1:1000 5%milk/TBS 60 Kda
Cyclin D1 H-295 (sc rabbit) 1:1000 5%milk/TBS 34 KDa
Cyclin D2 M20 (sc rabbit) 1:1000 5%milk/TBS 34 KDa
Cyclin E M20 (sc rabbit) 1:1000 5%milk/TBS 53 KDa
E2F1 Sc-251 (sc mouse) 1:1000 5%milk/TBS 55 KDa
P21CIP F5 (sc mouse) 1:1000 5%milk/TBS 21 KDa
P27KIP (M-197) sc776 rabbit 1:1000 5%milk/TBS 27 KDa
CDK4 C22 (sc rabbit) 1:1000 5%milk/TBS 34 KDa
CDK2 H-298 (sc rabbit) 1:1000 5%milk/TBS 34 KDa
PCNA PC-10 (sc. Mouse) 1:1000 5%milk/TBS 36 KDa
MDM2 Abcam 2A10 (mouse) 1:1000 5%milk/TBS 80 KDa(30-50 μg)
Cleaved Casapse 3 Cell sign (rabbit) #9664 1:1000 5%milk/TBS 18 KDa
Cdc27 C4 (sc mouse) 1:1000 5%milk/TBS 102 KDa
  1. TIMING: 1 week.