Table 3 MoFlo Instrument Settings for detection of DilC and PI signal.
From: A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells
Laser | Excitation wavelength & power | Band Pass Filters used for collection of emitted fluorescence (wavelength/power) |
|---|---|---|
Blue (PI) | 488 nm at 30 mWatts | Orange emission - 580/30 (from PI) |
| Â | Â | Red emission - 670/30 (from PI) |
Red (DilC) | 633 nm at 35 mWatts | Red emission - 670/20 (from DilC) |