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Table 2 MoFlo Instrument Settings the Flow Cytometry machine for detection of GFP, EdU and DAPI signals.

From: A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

Laser Excitation wavelength & power Band Pass Filters used for collection of emitted fluorescence (wavelength/power)
Blue 488 nm at 30 mWatts Green emission - 530/30 (from anti-GFP/Alexa Fluor 488)
Ultra violet 351 nm at 20 mWatts Blue emission - 450/65 (from DAPI)
Red 633 nm at 35 mWatts Red emission - 670/20 (from Alexa Fluor 647 azide linked to EdU)