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Table 2 MoFlo Instrument Settings the Flow Cytometry machine for detection of GFP, EdU and DAPI signals.

From: A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

Laser

Excitation wavelength & power

Band Pass Filters used for collection of emitted fluorescence (wavelength/power)

Blue

488 nm at 30 mWatts

Green emission - 530/30 (from anti-GFP/Alexa Fluor 488)

Ultra violet

351 nm at 20 mWatts

Blue emission - 450/65 (from DAPI)

Red

633 nm at 35 mWatts

Red emission - 670/20 (from Alexa Fluor 647 azide linked to EdU)