Analysis of different DNA dyes in pluripotent cells. Panel A: Comparison of FACs histograms of four different DNA dyes, staining of Jurkat cells, Human ESC and human IPS cells. We found that DAPI gave the best overall profile of the G1, S and G2/M phases of the cell cycle across different cell types described in the figure. Panel B: FACs histograms of DAPI staining of mouse ESC at day zero (undifferentiated pluripotent cells) and at different stages of differentiation using general differentiation cell culture conditions (Gelatin coated plates with 20% FCS in DMEM). Note the dramatic changes in the overall profile of the G1, S and G2/M phases of the cell cycle with differentiation.