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Table 1 Agonist-stimulated [35S] GTPγS binding measurements for the WT and Del α2B-ARs expressed in PC12 cells

From: Impaired desensitization of a human polymorphic α2B-adrenergic receptor variant enhances its sympatho-inhibitory activity in chromaffin cells

 

Basal

Agonist-stimulated

Des.

  

Control

UK14304

 
 

Cpm/min/15 μg

%

%

WT α2B-AR

2983,15 ± 09,74

240,01 ± 13,02

113,74 ± 18,40a

53

Del α2B-AR

5717,39 ± 13,02b

221,15 ± 14,94

208,47 ± 19,93b

6

  1. Agonist-stimulated [35S]GTPγS binding was determined in response to 10 μM UK14304 (agonist) in agonist-pretreated (UK14304) and in non-pretreated (Control) membranes. Des. = Desensitization.
  2. a p < 0.05 compared with control, b p < 0.05 compared with WT, n = 6 independent experiments.
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Cell Communication and Signaling

ISSN: 1478-811X