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Table 1 Agonist-stimulated [35S] GTPγS binding measurements for the WT and Del α2B-ARs expressed in PC12 cells

From: Impaired desensitization of a human polymorphic α2B-adrenergic receptor variant enhances its sympatho-inhibitory activity in chromaffin cells

  Basal Agonist-stimulated Des.
   Control UK14304  
  Cpm/min/15 μg % %
WT α2B-AR 2983,15 ± 09,74 240,01 ± 13,02 113,74 ± 18,40a 53
Del α2B-AR 5717,39 ± 13,02b 221,15 ± 14,94 208,47 ± 19,93b 6
  1. Agonist-stimulated [35S]GTPγS binding was determined in response to 10 μM UK14304 (agonist) in agonist-pretreated (UK14304) and in non-pretreated (Control) membranes. Des. = Desensitization.
  2. a p < 0.05 compared with control, b p < 0.05 compared with WT, n = 6 independent experiments.