Figure 3
Enhanced inhibition of nicotine-induced catecholamine secretion by the Del α 2B -AR in PC12 cells. In vitro catecholamine secretion from PC12 cells, expressing the WT α2B-AR (WT) or the Del α2B-AR (Del) and pretreated with 10 μM UK14304, in response to either 50 μM nicotine alone (Nicotine) or to 50 μM nicotine plus another challenge of 10 μM UK14304 (UK14304 + Nicotine). No differences between the two non-pretreated cell lines were observed (data not shown). *, p < 0.05, vs. WT/Nicotine, **, p < 0.05, vs. WT/UK14304 + Nicotine, n = 3 independent experiments.