Molecular mechanisms of gastric epithelial cell adhesion and injection of CagA by Helicobacter pylori

Table 4 Cell lines with reported resistance for phosphorylation/injection of CagA ^a

Cell line	Origin	Species	*H. pylori* strains used	Applied methods	References
Hek293	kidney	human	P1, P12, 26695, P310	Anti-PY WB/SI	[99]
GLC4	Lung	human	P1, P12	Anti-PY WB/IPA	[74]
CHOK1	Ovary	hamster	P1, P12	Anti-PY WB/IPA	[74]
MDCK	Kidney	dog	P1, P12	Anti-PY WB/IPA	[74]

^a Abbreviations: anti-PY WB (Westernblotting of infected cell lysates using several pan-phospho-tyrosine antibodies); IPA (in vitro phosphorylation assays of CagA with cell lysates to ensure that active kinases are present in the cells of interest); SI (synchronised infection assays: bacteria were centrifuged onto Hek293 cells to ensure proper contact of bacteria with these host cells).

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