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Table 3 Reported phosphorylation/injection of CagA in cell lines other than human a

From: Molecular mechanisms of gastric epithelial cell adhesion and injection of CagA by Helicobacter pylori

Species Cell line Origin H. pylori strains used Phospho-CagAb Applied methods References
Mouse J774.A1 blood P1, P12, G27, ATCC43526 +++ IP, 2-DE, Anti-PY WB, MS [96, 97]
Mouse Fibroblast embryo P12 + Anti-PY WB, CF [61]
Mouse SR 4987 bone marrow P1, P12 + Anti-PY WB/IPA [74]
Mouse L929 fibroblasts P1, P12 + Anti-PY WB/IPA [74]
Monkey Cos1 kidney P1, P12 + Anti-PY WB/IPA [74]
  1. a Abbreviations: 2-DE (two-dimensional protein gel electrophoresis); anti-PY WB (Westernblotting of infected cell lysates using several pan-phospho-tyrosine antibodies); IPA (in vitro phosphorylation assays of CagA with cell lysates to ensure that active kinases are present in the cells of interest); IF (localization by immunofluorescence), IP (immunoprecipitation); CF (cell fractionation); MS (mass spectrometry of precipitated CagAPY).
  2. b Intensity of CagA's tyrosine phosphorylation signals: +++ strong phosphorylation; ++ moderate phosphorylation; + weak phosphorylation.