Molecular mechanisms of gastric epithelial cell adhesion and injection of CagA by Helicobacter pylori

Table 3 Reported phosphorylation/injection of CagA in cell lines other than human ^a

Species	Cell line	Origin	*H. pylori* strains used	Phospho-CagA^b	Applied methods	References
Mouse	J774.A1	blood	P1, P12, G27, ATCC43526	+++	IP, 2-DE, Anti-PY WB, MS	[96, 97]
Mouse	Fibroblast	embryo	P12	+	Anti-PY WB, CF	[61]
Mouse	SR 4987	bone marrow	P1, P12	+	Anti-PY WB/IPA	[74]
Mouse	L929	fibroblasts	P1, P12	+	Anti-PY WB/IPA	[74]
Monkey	Cos1	kidney	P1, P12	+	Anti-PY WB/IPA	[74]

^a Abbreviations: 2-DE (two-dimensional protein gel electrophoresis); anti-PY WB (Westernblotting of infected cell lysates using several pan-phospho-tyrosine antibodies); IPA (in vitro phosphorylation assays of CagA with cell lysates to ensure that active kinases are present in the cells of interest); IF (localization by immunofluorescence), IP (immunoprecipitation); CF (cell fractionation); MS (mass spectrometry of precipitated CagA^PY).
^b Intensity of CagA's tyrosine phosphorylation signals: +++ strong phosphorylation; ++ moderate phosphorylation; + weak phosphorylation.

ISSN: 1478-811X