Molecular mechanisms of gastric epithelial cell adhesion and injection of CagA by Helicobacter pylori

Table 1 Characteristics of cag PAI-independent and cag PAI-dependent host cell adhesion factors^a

Bacterial factor	Reported host receptor	Cell systems used	H. pylori strains used	Applied methods	References
BabA	Fucosylated blood group antigens Leb, and H1	Human gastric tissue sections	P466, CCUG17875, A5, M019, 26695	RIA, Receptor displacement asssay, Scatchard analysis,	[14]
	Leb, H1, A, ALeb, and BLeb	-	P466, CCUG17875, J99	Overlay binding studies with radiolabelled glycoconjugates	[15]
BabB	Unknown	-	-	-	-
SabA	Sialylated dimeric Lewis x, Sialyl Lea antigen	Human and monkey gastric tissue sections	CCUG17875, J99, 26695, SM165, WU12	RIA and Scatchard analysis	[26]
	Laminin	-	J99	Glycoconjugate array binding studies	[30]
OipA	Unknown	AGS, KATO-III	B128, G27	Bacterial adherence assays	[37]
AlpA/B	Laminin	-	26695, SS1	Flow cytometry, Biacore binding studies, surface adherence assay	[33]
HopZ	Unknown	AGS	ATCC43504, 342, 326/01	AGS cell adhesion assays	[39]
CagA	β1 integrin	GE11 vs. GE11β1	P12	Y2H, PD with magnetic beads, FACS, Biacore binding studies	[82]
	Phosphatidylserine	AGS	NCTC11637	Binding studies, CF, AB blocking, IF	[83]
CagI	β1 integrin	-	-	Y2H, PD with magnetic beads, FACS	[82]
CagL	β1 integrin	AGS, GD25 vs. GD25β1, HeLa, mouse fibroblasts	P1, P12	Biacore binding studies, Peptide competition, cell adhesion assays, AB blocking, CF	[61, 81]
CagY	β1 integrin	GE11 vs. GE11β1	P12	Y2H, PD with magnetic beads, FACS	[82]

^a Abbreviations: AB (antibody); CF (cellular fractionation); FACS (Fluorescence-activated cell sorting); IF (co-localization by immunofluorescence); PD (pull-down experiments); RIA (Radioimmuno assay); Y2H (yeast two hybrid screen).

ISSN: 1478-811X