Figure 5
From: Abolished adherence alters signaling pathways in phorbol ester-induced human U937 cells
Immunoprecipitation of Pyk-2. The pMTH1-U937 (pMTH1) and asCD11b-U937 (asCD11b) cells were cultured in the absence and in the presence of 5 nM TPA for 24 h up to 72 h, respectively. Thereafter, the different populations were harvested, lyzed and after pre-absorbation incubated with sepharose-conjugated anti-Pyk-2. Precipitated Pyk-2 samples (IP Pyk-2) were then separated by 10% SDS-PAGE followed by Western blotting for Pyk-2 equal loading and for Pyk-2 phosphorylation by phosphotyrosine (P-Tyr).