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Figure 4 | Cell Communication and Signaling

Figure 4

From: Abolished adherence alters signaling pathways in phorbol ester-induced human U937 cells

Figure 4

Western blot analysis. The pMTH1-U937 (pMTH1) and asCD11b-U937 (asCD11b) cells were cultured in the absence and in the presence of 5 nM TPA for 24 h up to 72 h, respectively. Thereafter, the different populations were harvested, lyzed and 40 μg of total cellular protein was. For the expression of growth and differentiation markers, antibodies against p21, c-Jun and vimentin were applied. Kinase expression was tested for FAK, PYK-2, pp60src and MAP kinase, respectively. The unaltered expression level of β-actin serves as a loading control.

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