Figure 3
From: Abolished adherence alters signaling pathways in phorbol ester-induced human U937 cells
Analysis of apoptosis by internucleosomal DNA fragmentation. Untreated pMTH1-U937 (pMTH1 control) and untreated asCD11b-U937 (asCD11b control) were incubated with 5 nM TPA at a density of 5 × 104 cells/ml either in uncoated or in 2% (w/v) agarose-precoated cell culture dishes for 24 h, respectively. Following DNA extraction the samples were analyzed in a 2% agarose gel and visualized by UV illumination after ethidium bromide staining. The cell cycle histograms were analyzed in the Flomax program (Partec) and the percentage of the distinct cell cycle phase has been calculated using the MultiCycle cell cycle software (Phoenix Flow Systems Inc., San Diego, CA).