Figure 2
From: Abolished adherence alters signaling pathways in phorbol ester-induced human U937 cells
Cell cycle analysis of U937, pMTH1-U937 and asCD11b-U937 cells. U937 cells, pMTH1-U937 and asCD11b-U937 cells were incubated in 6-well plates at a density of 5 × 104 cells/ml in the absence or presence of 5 nM TPA for up to 72 h, respectively. At the time points indicated representative aliquots of each population were fixed in 70% ethanol, stained with a CyStain DNA kit and subjected to cell cycle analysis by flow cytometry. The percentage of cell cycle distribution was gated and calculated using the Multi-cycle software.