Characterization of Tet-regulated transgene expression in MCF-10A cells. (A, B) FACS analysis showing a typical (A) dose-response and (B) time course experiment. (C) MCF-10Atet cells stably transfected with pTET/HAhBRAF-GFP-bsr were induced with dox for 24 h followed by a wash-out of dox. Transgene expression was monitored by Western blotting. (D) MCF-10Atet cells were induced with dox for 24 h and either kept under dox treatment for additional 6 days (+ 6 days) or subjected to a dox wash-out (wo 6 days). Cells were lysed and Western blot analysis was performed using the indicated antibodies.