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Figure 1 | Cell Communication and Signaling

Figure 1

From: FLIP (Flice-like inhibitory protein) suppresses cytoplasmic double-stranded-RNA-induced apoptosis and NF-κB and IRF3-mediated signaling

Figure 1

FLIP-/- MEFs are more susceptible to cytoplasmic poly(I:C)-induced killing. (A) WT (open bars) and FLIP-/- (closed bars) MEFs were untreated, transfected with LF (8 μg/ml) + poly(I:C) (1.5, 3, 6 μg/ml) or treated with mFasL (1 ng/ml) for 6 hrs and assayed for viability using the Sytox green dye. Values represent the means of 3 wells in a single experiment. Similar results were obtained in two independent experiments. *p < 0.05 compared to WT (B) WT (open bars) and FLIP-/- (closed bars) MEFs were either untreated or pretreated with z-IETD-fmk or z-VAD-fmk (100 μM) for 1 hour and then treated with LF/poly(I:C) or mFasL (1 ng/ml) for 6 hrs. Viability was determined by homogeneous caspase assay. Values represent the means of 3 wells in a single experiment. *p < 0.05 compared to WT. Similar results were obtained in two independent experiments.

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