Figure 4
From: LiCl induces TNF-α and FasL production, thereby stimulating apoptosis in cancer cells
LiCl activates the extrinsic apoptosis pathway. HCT116 wild type and HCT116 p53-/- cells were incubated with 50 mM LiCl for the indicated time. (A.I) Cell lysates were prepared and 50 μg of protein were separated on a 10% SDS-PAGE gel. Proteins were transferred onto a PVDF membrane and probed with an antibody directed against Caspase-8, and against PCNA for loading control. (A.II) Cell lysates were prepared and the activity of Caspase-8 was determined with the Caspase-8 activity assay (Clonetech). (B) Cells were lysed and 50 μg of protein were separated on a 10% SDS-PAGE gel. Proteins were transferred onto a PVDF membrane and probed with an antibody directed against Caspase-10, and against PCNA for loading control.