Figure 2
From: Src mediates cytokine-stimulated gene expression in airway myocytes through ERK MAPK
PP1 inhibits cytokine expression and synthesis. Human airway myocytes were treated for 20 hours with 10 ng/ml IL-1β, TNFα and IFNγ in the presence of the 0.1% DMSO vehicle + 30 μM PP1. A-C. Relative multi-plex RT-PCR amplifying products of the predicted size for 18S rRNA (324 bp), IL-1β (291 bp), IL-6 (425 bp), and IL-8 (188 bp) with a graphical summary of expression normalized to 18S rRNA, n = 4 + SEM. Data is expressed as the % change from cytokine stimulated cultures (100%). D. Western analysis demonstrating intracellular pro-IL-1β immunoreactivity at 33 kDa, n = 5 + SEM. E-F. IL-6 or IL-8 secretion measured by ELISA, n = 3 + SEM; N.D. = not detected; * indicates significant difference from cytokine stimulated cultures, p < 0.05.