Figure 1
From: Src mediates cytokine-stimulated gene expression in airway myocytes through ERK MAPK
Effect of cytokine stimulation on Src tyrosine kinase activity in airway myocytes. A. Cultures were treated at the times indicated with 10 ng/ml IL-1β, TNFα and IFNγ in the presence of the 0.1% DMSO vehicle (closed circles) or 30 μM PP1 (open circles). In vitro Src kinase assays were performed using the synthetic peptide p34cdc2 (6-20) as the substrate, n = 3 ± SEM. B. Cultures were treated for 5 minutes with 10 ng/ml IL-1β, TNFα and IFNγ in the presence of the 0.1% DMSO vehicle or 30 μM PP1. Immune-complex kinase assays were performed as above in Src immunoprecipitates. Data are expressed as the fold change relative to the absence of stimulus, n = 4 ± SEM. * indicates significant difference from unstimulated or PP1 treated cultures, p < 0.05.