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Figure 5 | Cell Communication and Signaling

Figure 5

From: Stoichiometry and intracellular fate of TRIM-containing TCR complexes

Figure 5

Colocalization of TRIM with CD3ε and ζ 1 hour post-αβTCR activation. (A) Human peripheral blood T cells were incubated with the IgM anti-CD3ε mAb 2Ad2a2 for 1 hour or left unstimulated. Cells were fixed and the subcellular localization of TRIM and ζ was determined using the antibodies TRIM-7 mAb (top) and anti-ζ mAb 6B10.2 (bottom). Secondary staining was done with a Texas red-labeled donkey anti-mouse antiserum. (B) Cells were stimulated as in (A), then fixed and the subcellular localization of ζ detected using FITC-labelled anti-ζ mAb 6B10.2. CD3ε was visualized using Texas Red-labelled goat anti-mouse IgM (left) and a biotinylated TRIM-4 mAb followed by Texas Red-coupled streptavidin were used to detect TRIM (right).

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