TRIM is part of the γδTCR complex. (A) The human γδ T cell line Peer (left) and a human Vγ9Vδ2 clone (right) were pervanadate-stimulated and lysed in digitonin. γδTCRs were purified with anti-phosphotyrosine antibodies followed by phenylphosphate elution. TCRs were separated by BN-PAGE and immunoblotted against ζ. Ferritin was used as a marker protein as in figure 1. (B) Peer γδ and Jurkat αβ T cells were lysed and immunoprecipitations performed with antibodies recognizing the indicated proteins (lanes 1 - 5 and 7 - 11). Purified proteins, as well as lysates (lanes 6 and 12), were separated by non-reducing SDS-PAGE. TRIM and ζ were detected by immunoblotting.