Figure 1
From: Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus
Expression of specific gene markers. RT-PCR analysis was performed using total RNA isolated from mononucleated cells cultured in proliferation medium and mononucleated cells cultured in osteoblast and osteoclast differentiation medium. (A) Gapdh (717 bp) was used as a positive control. (B) The low expression of Opn (234 bp) in undifferentiated mononucleated cells while high expression of Opn (234 bp) indicates differentiation into mature osteoblasts. (C) Catk (350 bp) expression shows osteoclast differentiation. Equal volumes of the products were separated in agarose gels and stained with ethidium bromide.