Figure 4

IL-27 stimulation leads to STAT1 phosphorylation and induces STAT1-dependent protein expression in primary hepatic stellate cells. A: Primary rat HSC were isolated as described in the Materials and Methods section and were treated with murine IL-27 (30 ng/mL) or with murine IFNγ (30 ng/mL) at day 5 after isolation for the times indicated. Phosphorylation of STAT1 and STAT3 was monitored by Western blot analysis as described for Figure 2. B: Western blot analysis monitoring upregulation of STAT1 and MxA protein expression upon stimulation of primary rat HSC with 30 ng/mL IL-27 or IFNγ for up to 24 h. Expression levels of PLCγ are provided to compare protein loading in the individual samples.