IL-27 mediates a STAT1 response in human LX-2 cells. A: Real-time PCR analysis of total cellular mRNA monitoring the expression of the STAT3-dependent gene SOCS3 in LX-2 cells after stimulation with IL-27 (30 ng/mL) for the times indicated. As a control, the cells were stimulated with IL-6/sIL6R (30 ng/mL/500 ng/mL) for 60 min. Beta-actin was used as internal reference to normalize the target transcripts. Standard deviations of a triplicate experiment are given. B: Western blot analysis monitoring upregulation of the STAT1-dependent STAT1, IRF-1 and MxA protein expression upon stimulation of LX-2 cells with 30 ng/mL IL-27 or IFNγ for up to 24 h. Expression levels of PLCγ are provided to compare the protein amount in the samples.