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Figure 2 | Cell Communication and Signaling

Figure 2

From: Interleukin-27 acts on hepatic stellate cells and induces signal transducer and activator of transcription 1-dependent responses

Figure 2

IL-27 treatment induces STAT1 and STAT3 phosphorylation in human hepatic stellate cells. A: LX-2 cells were stimulated with each 30 ng/mL IL-27, IFNγ and IL-6 together with sIL-α (500 ng/mL) for the indicated times. After lysis, cellular proteins were resolved by SDS-PAGE and tyrosine phosphorylation of STAT1 and STAT3 was detected by Western blot analysis using phospho-specific antibodies for pY701-STAT1 and pY705-STAT3. STAT protein expression was monitored by stripping and re-probing the blot with antibodies directed against total STAT1 and STAT3. Equal loading of the samples was assessed by probing the blot with an antibody against tubulin. B: LX-2 cells were stimulated with 30 ng/mL IL-27, IL-6 or IFNγ for the times indicated and nuclear extracts were prepared. These were analyzed by EMSA and STAT3/3, STAT3/1 and STAT1/1 dimer species were visualized by autoradiography.

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