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Figure 3 | Cell Communication and Signaling

Figure 3

From: The heparan sulfate co-receptor and the concentration of fibroblast growth factor-2 independently elicit different signalling patterns from the fibroblast growth factor receptor

Figure 3

Dose-response of DNA synthesis and dual phosphorylation of p42/44 MAPKstimulated by FGF-2 Quiescent serum-starved Rama 27 fibroblasts were stimulated with FGF-2 and EGF. (A, B) DNA synthesis was determined by the incorporation of [3H] thymidine into the DNA of quiescent Rama 27 fibroblasts 18 h after the addition of growth factor (see Methods) and results are the mean ± SD of triplicate wells of two experiments. (A) Dose response curve for FGF-2. (B) Stimulatory effect of 3 ng/mL EGF in the absence or presence of 300 ng/mL FGF-2. BSA is the negative control with no growth factor. (C) Dose-response of dual phosphorylation of p42/44 MAPK in quiescent Rama 27 fibroblasts stimulated with the indicated doses of FGF-2 for 15 min. The doubly phosphorylated Thr 183/202/Tyr 185/204 forms of p42/44 MAPK (P- p42/44 MAPK) were detected with a monoclonal antibody (see Methods). The same blot was re-probed with anti-actin to show the level of loading of the gel.

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