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Figure 1 | Cell Communication and Signaling

Figure 1

From: The heparan sulfate co-receptor and the concentration of fibroblast growth factor-2 independently elicit different signalling patterns from the fibroblast growth factor receptor

Figure 1

HS effect on the kinetics of dual phosphorylation of p42/44 MAPK(A) DNA synthesis was determined in chlorate-treated quiescent serum-starved Rama 27 fibroblasts by the incorporation of [3H] thymidine into DNA 18 h after the addition of growth factor (see Methods), as follows: control, no addition, FGF, (0.3 ng/mL), heparin (10 ng/mL). The results are the mean ± SD of triplicate wells of two experiments. (B) Control or chlorate-treated quiescent Rama 27 fibroblasts were stimulated with 0.3 ng/mL FGF-2 (upper panel) or 0.3 ng/mL FGF-2 and 10 ng/mL heparin (lower panel) for 0 min to 60 min and the doubly phosphorylated Thr 183/202/Tyr 185/204 forms of p42/44 MAPK (P-p42/44 MAPK) were detected with a monoclonal antibody (see Methods). The same blot was re-probed with anti-actin to show the level of loading of the gels.

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Cell Communication and Signaling

ISSN: 1478-811X