Spatial learning was impaired after THC treatment. C57Bl/6 female mice were either fed with food supplemented with THC-rich or CBD-rich plant extracts or a control diet. Spatial memory was tested after 6 weeks of treatment. THC mice were slower in finding the hidden platform over the whole acquisition period and during the reversal learning, with regard to both latency (A) and distance (B) to platform. All three groups performed at the same level when the platform was made visible on day 6 to test for possible visual impairments and the general ability to perform the task. A Rotarod test to measure general locomotor function and fitness was performed on day 7. The THC group performed better than CTR (C); * p ≤ 0.05.