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Figure 8 | Cell Communication and Signaling

Figure 8

From: Human uterine leiomyoma-derived fibroblasts stimulate uterine leiomyoma cell proliferation and collagen type I production, and activate RTKs and TGF beta receptor signaling in coculture

Figure 8

Detection of activated TGF-βRI and downstream proteins, p-Smad-2 and -3 at 48 h. (A) Immunoprecipitation of TGF- βRI, blotted with anti-phospho-serine. There was increased expression of phospho-serine in immunoprecipitated TGF-βRI in cocultures of UtLM/FB compared to cultures of FB or UtLM cells alone. There were no significant differences in total (t)-TGF-βRI expression between the culturing conditions. (b) Western blots of p-Smad-2 and p-Smad-3 and t-Smad-2 and t-Smad-3. Densitometric analyses showed a significant increase in the expression of phospho-Smad-2 (p = 0.001) and phosho-Smad-3 (p = 0.05) in UtLM/FB compared to UtLM cells or FB cultured alone. There was no significant difference in t-Smad-2 expression between UtLM/FB and UtLM cells cultured alone. All error bars represent SEM.

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