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Figure 3 | Cell Communication and Signaling

Figure 3

From: Human uterine leiomyoma-derived fibroblasts stimulate uterine leiomyoma cell proliferation and collagen type I production, and activate RTKs and TGF beta receptor signaling in coculture

Figure 3

ECM Proteins. (A) Western blots. Collagen-I and IGF-BP-3 protein secretion in media of UtLM cells or UtSCM cultured in the presence (UtLM/FB or UtSMC/FB) or absence of FB. (B) Densitometry. Collagen I was significantly (p = 0.02) increased in the media of UtLM/FB cells compared to UtLM cells cultured alone. There was no difference between UtSMC versus UtSMC cocultured with FB. There was a significant (p = 0.02) increase in the expression of IGF-BP-3 in the cocultured UtLM cells compared to UtLM cells or FB cultured alone. Furthermore, IGF-BP-3 levels were also significantly higher in the cocultured UtSMC than UtSMC and FB cultured alone, but the level was minimal. There was a limited expression of IGF-BP-4 compared to IGF-BP-3 for both UtLM cells and UtSMC under cocultured or single cultured conditions. However, there was a significant (p = 0.02) increase in IGF-BP-4 expression in the cocultured UtSMC compared to UtSMC cultured alone. All error bars represent SEM.

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Cell Communication and Signaling

ISSN: 1478-811X