Immunohistochemistry of differentially expressed proteins. A-B: Expression of SOCS3 and C-D: BAX in the control and 6 hrs after optic nerve crush. SOCS3 was expressed throughout the retina and appeared increased in Muller cells (arrowheads) after optic nerve injury. Increased BAX labeling after optic nerve injury was particularly evident in the ganglion cell layer (arrows), consistent with pending programmed cell death of RGCs. Red = target antibody, Blue = DAPI.