MP1 depletion decreases membrane activity and the number of focal adhesions at the wound edge. A) DU145 cells transfected with control or MP1 siRNA were wounded as described. Approximately 2 hours post-wounding, cells were imaged at 1 second intervals for 5 min for kymograph analysis. For each cell, 6-7 kymographs were generated (shown by numbered lines); 5-7 cells per condition were analyzed. Boxes around the number indicate the representative kymograph shown. B) Control and MP1 siRNA transfected cells plated on coverslips and imaged for a wound-healing assay were fixed and stained for vinculin to mark focal adhesions. Average number and size of FA occurring at the wound edge were quantified using ImageJ (chart). C) Size distribution of all focal adhesions in the cells imaged for B. MP1 depleted cells have fewer focal adhesions at the wound edge.