ERK activation is not required for DU145 migration on FN, but is required for migration on FN induced by serum. A) Results of DU145 wound healing on FN. Shown are averaged results from 4 experiments; error bars represent standard error of the mean (S.E.M.). Migration in response to fibronectin alone was not affected by UO126. Serum and EGF stimulated significant (p < 0.0001) increases in migration, that were significantly inhibited (p < 0.0001 for serum, p = 0.0004 for EGF) by UO126 treatment. B) Sample images from wound healing assay; for each condition, six grid squares were imaged at 0 and 10 h post-wounding, and wound area (outlined in white) was measured using ImageJ software (NIH).