Figure 4

Visualization of GPCRs by peptide- and protein-tag technologies (A; above: Lumio™-tag technology and below: SNAP™-tag technology). Membrane and subcellular localization of human Y₁ receptor C-terminally fused to the tetracysteine motif in HEK293 cells and labeled with the membrane-permeable FlAsH dye, which fluoresces green after binding to the motif (B; bar represents 10 μm). Cellular distribution of hY₁R C-terminally modified by the mutant hAGT in HEK293 cells and labeled with the membrane-permeable, red fluorescent BG-TMR-Star dye (C; bar represents 10 μm).