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Figure 1 | Cell Communication and Signaling

Figure 1

From: Gamma-secretase inhibition combined with platinum compounds enhances cell death in a large subset of colorectal cancer cells

Figure 1

Expression level and size heterogeneity of Notch fragments detected in CRC cells. A Western blot detection of Notch fragments in total cell RIPA lysates. 100 μg of total cell protein extracts were subjected to SDS-PAGE and immunoblotting to determine the abundance and sizes of C-terminal fragments of Notch1 (NICD). Results from 16 CRC lines are shown. The heterogeneity in the level of NICD expression and the presence of several NICD fragments of variable length is notable. B Comparison of NICD bands obtained with total cell RIPA lysates (R) and lysates obtained by harvesting cells with boiling SDS-PAGE sample buffer (S). 20 CRC lines were analysed in total. Results from 4 CRC lines are shown as an example. Both protein extraction methods result in very similar NICD band patterns. C Size heterogeneity of immunoprecipitated NICD fragments. NICD fragments were precipitated with a polyclonal antibody (sc-6014-R) directed against the C-terminus of Notch1 and, after extended SDS-PAGE on a large 7% gel, immunoblotted with a mouse mAb (N6786) directed against an epitope in the cdc10-NCR region of Notch1.

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Cell Communication and Signaling

ISSN: 1478-811X