Skip to main content

Advertisement

Figure 3 | Cell Communication and Signaling

Figure 3

From: Serine phosphorylation regulates paxillin turnover during cell migration

Figure 3

Cells expressing EGFP-paxillin or EGFP-S188/190A paxillin have similar adhesion but different spreading and migration rates on collagen. A) NBT-II cells transfected with EGFP-paxillin or EGFP-S188/190A were allowed to adhere on dishes coated with collagen at the indicated concentrations for 1 h at 37°C. Absorbance values were obtained by crystal violet uptake assay and expressed as percent of maximal adhesion. Values represent the mean of three independent experiments carried out in triplicate ± SEM (left panel). Expression of endogenous and transfected paxillin is shown on the right panel. B) Cell spreading was quantified by allowing cells to adhere onto collagen coated plates (10 μg/ml) for the indicated times. The percentage of spread cells among the GFP-positive population was then calculated for each time point. Values represent the mean of three independent experiments carried out in triplicate ± SEM (left panel). Expression of endogenous and transfected paxillin is shown on the right panel. C) NBT-II cells transfected with either plasmids were plated on plastic or collagen-coated plates (10 μg/ml) for 4 h. Cell motility was evaluated by tracking individual cells for 10 h using the Metamorph software. Mean migration rate of cells is expressed as μm/h. Values represent the mean of three independent experiments ± SEM (left panel). Expression of endogenous and transfected paxillin is shown on the right panel. **, p < 0.01

Back to article page